This is exactly the source of energy exploited by the fission of high-mass nuclei (i.e. nuclear power).
You start with a heavy nucleus (say $^{235}\mathrm{U}$) and add a neutron. What you get out is two lighter nuclei (often, but not always, krypton-92 and barrium-141) and several neutrons:
$$ ^{235}\mathrm{U} + n \to ^{92}\mathrm{Kr} ^{141}\mathrm{Ba} + n + n + n \,.$$
The mass of all of these bits can be measured to high precision, and the mass of the reactants is larger than the mass of the products.
The differences is that there is more internal energy in the uranium than the total internal energy in the krypton and barium.
The extra energy shows up as kinetic energy (mostly of the product neutrons) and is converted to thermal energy in the moderator, used to heat water, which runs a turbine and powers your iphone (or what every).
Short-short answer: Yes, internal energy is mass (though you need the factor of $c^2$ to put it in units of kilograms).
Aside (on account of your previous question): No, that doesn't mean that it is better to talk about "relativistic mass" because internal energy includes binding as well as motion. It just means that the mass of a composite system is not necessarily the same as the mass of it's constituents.
Homework: Determine how much mass 1 kilogram of water gains when heated (at STP) from freezing to boiling.