Let's perform two simple calculations and then formulate the relative questions.
By checking Wikipedia, one can know the mass of neutron and proton: $$M_{neutron}=939.5654133(58)MeV/c^2=1.674927471(21)×10^{−27} kg$$ $$M_{proton}=938.2720813(58) MeV/c^2=1.672621898(21)×10^{−27} kg$$ and the neutron (proton) is composed of two down (up) quarks and one up (down) quarks. Also by checking Wikipedia, one can know the mass of up quark and down quark, respectively. $$M_{up-quark} \approx 2.3MeV/c^2 \approx 4.1 \times 10^{-30} kg \qquad M_{down-quark} \approx 4.8Mev/c^2 \approx 8.6 \times 10^{-30} kg $$ One can see immediately that $$M_{neutron} >> 2\times M_{down-quark}+1 \times M_{up-quark}$$ $$M_{proton} >> 2\times M_{up-quark}+1 \times M_{down-quark}$$ One can also see only one percent of the total mass of the neutron/proton is contributed by their building blocks (up- and down- quarks)!So my first question is where is the mass of neutron?
We can perform the similar calculations for Helium-4, which is composed of two protons and two neutrons. By checking Wikipedia, one can also know the mass of helium-4. But we have the following inequality: $$M_{helium-4}=6.645 \times 10^{-27}kg<2\times M_{proton}+2\times M_{neutron}=6.695\times 10^{-27}kg$$ So my second question is: what's the additional mass of helium-4 atom?
My final question is about what's the difference between calculation one (far greater than their constituents )and calculation two (lesser than their constituents)?